Indian Journal of Human Genetics
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Year : 2013  |  Volume : 19  |  Issue : 1  |  Page : 14-17

Rapid detection of chromosome X, Y, 13, 18, and 21 aneuploidies by primed in situ labeling/synthesis technique

Department of Reproductive Biology, AIIMS, New Delhi, India

Correspondence Address:
Ashutosh Halder
Department of Reproductive Biology, AIIMS, New Delhi - 110 029
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Source of Support: Indian Council of Medical Research and AIIMS, New Delhi, India,, Conflict of Interest: None

DOI: 10.4103/0971-6866.112877

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Aims and Objective: Primed in situ labeling/synthesis (PRINS) technique is an alternative to fluorescent in situ hybridization for chromosome analysis. This study was designed to evaluate the application of PRINS for rapid diagnosis of common chromosomal aneuploidy. Materials and Methods : We have carried out PRINS using centromere specific oligonucleotide primers for chromosome X, Y, 13, 18 and 21 on lymphocyte metaphase and interphase cells spread. Specific primer was annealed in situ, followed by elongation of primer by Taq DNA polymerase in presence of labeled nucleotides. Finally, reaction was stopped and visualized directly under fluorescent microscope. Results: Discrete centromere specific signals were observed with each primer. Conclusion: PRINS seems to be a rapid and reliable method to detect common chromosome aneuploidy in peripheral blood lymphocyte metaphase and interphase cells.

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